Biotech 1402 - Methods In Biotechnology

News: Added analysis routine for lysozyme lab (4/27).

What's due when?

Write-up for lysozyme is due by the end of the day on Thursday, 4/30. See below for the template.

Every other assignment is now past due. You may turn in any past due assignment up to the day of the final for HALF CREDIT. These will not be graded or returned.

FINAL REVIEW:  There is none scheduled for this class. Email or stop by if you have any questions.

FINAL EXAM:  Wednesday, May 6, 1-2:50 PM

Syllabus (2009)

Schedule (2009)

   Schedule, mod 1-21-09

Conversion from Seidman And Moore Ed. 2 to Ed. 1

Student information sheet

EXAM CORRECTIONS

Link to NCBI website (for references)

Lectures:

Introduction to Methods

   Biomedical Research Timeline

Accuracy and Precision exercise

Acid/base problem set

Significant figures practice

Math Quiz

   Saved in MSWord97

Acid/Base lecture

pKA's of organic acids

Solutions and Dilutions

Solutions and Dilutions, part 2

Molarity calculator

Large molecule problem set

Statistical methods

   Spreadsheet to practice statistics

Spectrophotometry

   "A classic instrument"

Protein Purification

   Example of protein purification

Centrifugation

Dialysis

   Pierce Dialysis Handout

Structure of Proteins

   Amino acids

   Amino acid problem set

Immunochemistry

   Updated, 2009

Electrophoresis

Enzymes

   Enzyme Kinetics Tutorial

cGMP

   GMP video

   GMP terminology

Special Topic: Influenza

   Chiron's Vaccine Fiasco

Laboratory Manual Updates:

Safety Lecture

Safety Lecture, 2009 update

TLC website

TLC movie

Biotech safety agreement

Page 1: The lab notebook

Page 2: Lab report guidelines

Page 3: Sample lab report

Page 9: How to Work with Excel

   Working with Excel rtf (use if you can't open the other file)

Page 9a: Excel Example

   Excel example rtf

Lab Assignment 1

   Assignment 1 rtf (use if you can't open the other file)

   Graphing assignment 1 (rev)

Lab Assignment 2

   Assignment 2 rtf

Phosphate buffer prep and Micropipette Lab (pp 29-49)

Buffer preparation problems

Neutral Red Spectrophotometry, lab protocol

Class data on pipetting

Dye spectra

Pipetting skills check

Rabbit IgG Purification and Characterization

   Overview

   REVISED Overview

   Saturated Ammonium Sulfate Precipitation

   REVISED SAS protocol

      Handout:  SAS SOP

   Dialysis protocol

      Dialysis protocol, rev 2009

   Column Chromatography

      Revised chromatography SOP

      Chromatography SOP, Revision 3

   Spectrophotometry, rev

      Handout:  spectrophotometry of proteins

   ELISA

      Revision, 2009

      Plate Diagram

      ELISA Analysis

   Electrophoresis

Lysozyme enzyme activity

   Rate data analysis lysozyme

   Rate data analysis revised -- turn this one in.

Lab 8: GMP Popcorn lab

     Objective

     Production flow diagram, blank

     Material control instructions

          Mat Con report

     Production instructions

          Prod - Batch record

          Prod - Cleaning SOP and log

          Prod - Spec Sheets

     QA instructions

     QC instructions

     Write-up